Publications

Legionella and beyond (2000-)
  1. Kubori T, Bui XT, Hubber A and Nagai H (2017) Legionella RavZ Plays a Role in Preventing Ubiquitin Recruitment to Bacteria-Containing Vacuoles. Front. Cell. Infect. Microbiol. 7:384. doi: 10.3389/fcimb.2017.00384
  2. Hubber A, Kubori T, Coban C, Matsuzawa T, Ogawa M, Kawabata T, Yoshimori T, Nagai H. Bacterial secretion system skews the fate of Legionella-containing vacuoles towards LC3-associated phagocytosis. Sci Rep. 2017 Mar 20;7:44795. doi: 10.1038/srep44795.
  3. Maita C, Matushita M, Okubo T, Matsuo J, Miyake M, Nagai H, Yamaguchi H. Draft Genome Sequences of Legionella pneumophila JR32 and Lp01 Laboratory Strains Domesticated in Japan. Genome Announc 4, (2016). doi: 10.1128/genomeA.00791-16.
  4. Hirayasu K, Saito F, Suenaga T, Shida K, Arase N, Oikawa K, Yamaoka T, Murota H, Chibana H, Nakagawa I, Kubori T, Nagai H, Nakamaru Y, Katayama I, Colonna M and Arase H. Microbially cleaved immunoglobulins are sensed by the innate immune receptor LILRA2. Nat. Microbiol. 1, 16054 (2016). doi: 10.1038/nmicrobiol.2016.54
  5. Kubori T,  *Nagai H. The Type IVB secretion system: an enigmatic chimera. Curr Opin Microbiol 29, 22-29 (2016). doi: 10.1016/j.mib.2015.10.001
  6. 永井宏樹、病原菌の侵入・増殖と宿主真核細胞の攻防、実験医学 (羊土社), 33, 2778-2783 (2015).
  7. Kuroda T, Kubori T, Thanh Bui X, Hyakutake A, Uchida Y, Imada K, *Nagai H. Molecular and structural analysis of Legionella DotI gives insights into an inner membrane complex essential for type IV secretion. Sci Rep 5, 10912 (2015). doi: 10.1038/srep10912
  8. Kubori T, Hubber AM, *Nagai H. Hijacking the host proteasome for the temporal degradation of bacterial effectors. Methods Mol Biol 1197, 141-152 (2014). doi: 10.1007/978-1-4939-1261-2_8
  9. Nagai H. The host-pathogen interaction of Legionella pneumophila. Nihon Saikingaku Zasshi 69, 503-511 (2014).
  10. 永井宏樹、レジオネラ症とその病原メカニズムをめぐる新展開、呼吸器内科 (科学評論社), 26, 9-15 (2014).
  11. Kubori T, Koike M, Bui XT, Higaki S, Aizawa SI, *Nagai H. Native structure of a type IV secretion system core complex essential for Legionella pathogenesis. Proc Natl Acad Sci U S A 111, 11804-11809  (2014). doi: 10.1073/pnas.1404506111
  12. Hubber A, Arasaki K, Nakatsu F, Hardiman C, Lambright D, De Camilli P, Nagai H, Roy CR. The machinery at endoplasmic reticulum-plasma membrane contact sites contributes to spatial regulation of multiple legionella effector proteins. PLoS Pathog 10, e1004222 (2014). doi: 10.1371/journal.ppat.1004222
  13. Ishida K, Sekizuka T, Hayashida K, Matsuo J, Takeuchi F, Kuroda M, Nakamura S, Yamazaki T, Yoshida M, Takahashi K, Nagai H, Sugimoto C, Yamaguchi H. Amoebal Endosymbiont Neochlamydia Genome Sequence Illuminates the Bacterial Role in the Defense of the Host Amoebae against Legionella pneumophila. PLoS One 9, e95166 (2014). doi: 10.1371/journal.pone.0095166
  14. Hubber A, Kubori T, *Nagai H. Modulation of the ubiquitination machinery by legionella. Curr Top Microbiol Immunol 376, 227-247 (2013). doi:10.1007/82_2013_343
  15. Hori JI, Pereira MS, Roy CR, *Nagai H, Zamboni DS. Identification and functional characterization of K(+) transporters encoded by Legionella pneumophila kup genes. Cell Microbiol 15, 2006-2019 (2013). doi:10.1111/cmi.12168
  16. Matsuo J, Nakamura S, Ito A, Yamazaki T, Ishida K, Hayashi Y, Yoshida M, Takahashi K, Sekizuka T, Takeuchi F, Kuroda M, Nagai H, Hayashida K, Sugimoto C, Yamaguchi H., Protochlamydia induces apoptosis of human HEp-2 cells through mitochondrial dysfunction mediated by chlamydial protease-like activity factor. PLoS ONE 8(2): e56005. doi:10.1371/journal.pone.0056005 (2013). doi:10.1371/journal.pone.0056005
  17. Nagai H, Kubori T. Purification and characterization of legionella u-box-type e3 ubiquitin ligase. Methods Mol Biol 954, 347-354 (2013). doi:10.1007/978-1-62703-161-5_21
  18. Tomoko Kubori and Hiroki Nagai. Bacterial effector-involved temporal and spatial regulation by hijack of the host ubiquitin pathway. Front. Microbiol., 2:145. Epub 2011 Jul 4 (2011). doi: 10.3389/fmicb.2011.00145
  19. Hiroki Nagai and Tomoko Kubori. Dot/Icm type IVB secretion systems of Legionella and other gram-negative bacteria. Front. Microbiol., 2:136. Epub 2011 Jun 2 (2011). doi: 10.3389/fmicb.2011.00136
  20. Tomoko Kubori, Naoaki Shinzawa, Hirotaka Kanuka and Hiroki Nagai, Legionella metaeffector exploits host proteasome to temporally regulate cognate effector. PLoS Pathogens, 6, 12, e1001216 (2010).　doi:　 10.1371/journal.ppat.1001216
    Covered as a research highlight in Nat. Rev. Microbiol. 9, 80-81 (February 2011) | doi:10.1038/nrmicro2509
  21. Noboru Nakano, Tomoko Kubori, Miki Kinoshita, Katsumi Imada and Hiroki Nagai, Crystal Structure of Legionella DotD: insights into relationship between type IVB and type II/III secretion systems. PLoS Pathogens 6, 10, e1001129 (2010). doi:10.1371/journal.ppat.1001129
    Covered as a "100 selected papers" in Annual Report 2010-2011 of Osaka University
  22. 永井宏樹、病原細菌のIV 型分泌系、日本細菌学雑誌(日本細菌学会)、65：379–386，(2010).
  23. 永井宏樹、レジオネラの宿主細胞内動態とエフェクター、実験医学 (羊土社)、27, 1542-1547 (2009).
  24. Tomoko Kubori, Akihiro Hyakutake and Hiroki Nagai.  Legionella translocates an E3 ubiquitin ligase that has multiple U-boxes with distinct functions. Mol. Microbiol. 67:1307–1319 (2008). DOI: 10.1111/j.1365-2958.2008.06124.x
  25. Hiroki Nagai, Eric D. Cambronne, Jonathan C. Kagan, Juan C. Amor, Richard A. Kahn and Craig R. Roy.  A C-terminal translocation signal required for Dot/Icm-dependent delivery of the Legionella RalF protein to host cells.  Proc. Natl. Acad. Sci. USA. 102: 826-831 (2005). doi:10.1073/pnas.0406239101
  26. Juan C. Amor, Jennifer Swails, Craig R. Roy, Hiroki Nagai, Alyssa Ingmundson, Xiaodong Cheng and Richard A. Kahn.  The structure of RalF, an ARF guanine nucleotide exchange factor from Legionella pneumophila, reveals the presence of a cap over the active site.  J. Biol. Chem, 280: 1392-1400 (2005). doi:10.1074/jbc.M410820200
  27. 永井宏樹、病原細菌のIVB型分泌系と宿主細胞内増殖、蛋白質核酸酵素 (共立出版)、50:44-49 (2005)
  28. 永井宏樹、レジオネラと宿主細胞の相互作用、化学療法の領域 (医薬ジャーナル社)、21:1631-1636 (2005)
  29. 永井宏樹、レジオネラの感染・増殖の機序を知る—IV型分泌系により宿主細胞中へ輸送されるエフェクターの探索、分子呼吸器病 (先端医学社)、9:490-491 (2005)
  30. Hiroki Nagai and Craig R. Roy. Show me the substrates: modulation of host cell function by type IV secretion systems. Cell. Microbiol. 5: 373-383 (2003). DOI: 10.1046/j.1462-5822.2003.00285.x
  31. Hiroki Nagai, Jonathan C. Kagan, Xinjun Zhu, Richard A. Kahn, and Craig R. Roy. A bacterial guanine nucleotide exchange factor activates ARF on Legionella phagosomes. Science 295: 697-682 (2002). DOI: 10.1126/science.1067025
    Covered as a research highlight in Nat. Rev. Mol. Cell Biol. 3, 148 (March 2002) | doi:10.1038/nrm771
  32. 永井宏樹、レジオネラ・ニューモフィラ、細菌毒素ハンドブック(櫻井純、本田武司、小熊恵二編　サイエンスフォーラム)、pp 547-549. (2002).
  33. Hiroki Nagai and Craig R. Roy. The DotA protein from Legionella pneumophila is secreted by a novel process that requires the Dot/Icm transporter. EMBO J. 20: 5962-5970 (2001). doi: 10.1093/emboj/20.21.5962
  34. Jorn Coers, Jonathan C. Kagan, Miguelina Matthews, Hiroki Nagai, Deborah M. Zuckman and Craig R. Roy. Identification of Icm protein complexes that play distinct roles in the biogenesis of an organelle permissive for Legionella pneumophila intracellular growth. Mol. Microbiol. 38: 719-736 (2000). DOI: 10.1046/j.1365-2958.2000.02176.x

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